In Vivo Cellular-Level 3D Imaging of Peripheral Nerves Using a Dual-Focusing Technique for Intra-Neural Interface Implantation.
Adv Sci (Weinh)
; 9(3): e2102876, 2022 01.
Article
in En
| MEDLINE
| ID: mdl-34845862
ABSTRACT
In vivo volumetric imaging of the microstructural changes of peripheral nerves with an inserted electrode could be key for solving the chronic implantation failure of an intra-neural interface necessary to provide amputated patients with natural motion and sensation. Thus far, no imaging devices can provide a cellular-level three-dimensional (3D) structural images of a peripheral nerve in vivo. In this study, an optical coherence tomography-based peripheral nerve imaging platform that employs a newly proposed depth of focus extension technique is reported. A point spread function with the finest transverse resolution of 1.27 µm enables the cellular-level volumetric visualization of the metal wire and microstructural changes in a rat sciatic nerve with the metal wire inserted in vivo. Further, the feasibility of applying the imaging platform to large animals for a preclinical study is confirmed through in vivo rabbit sciatic nerve imaging. It is expected that new possibilities for the successful chronic implantation of an intra-neural interface will open up by providing the 3D microstructural changes of nerves around the inserted electrode.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sciatic Nerve
/
Imaging, Three-Dimensional
/
Tomography, Optical Coherence
/
Electrodes, Implanted
Limits:
Animals
Language:
En
Journal:
Adv Sci (Weinh)
Year:
2022
Document type:
Article